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Home / Baking Basics
pouring homemade buttermilk into a glass jar.

How to Make Buttermilk

Becky Hardin

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Updated: August 22, 2025
4.80 from 5 votes

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buttermilk pin.
pouring homemade buttermilk into a glass jar.
buttermilk pin.

If you’ve ever started a recipe and realized you don’t have buttermilk on hand, don’t worry! My homemade buttermilk recipe is the easiest kitchen trick every baker should know. With just milk and an acid (vinegar or lemon juice), you can whip up a quick, easy buttermilk alternative in minutes. It works as the perfect buttermilk substitute for biscuits, pancakes, muffins, fried chicken, and more. Not only does this DIY version save a trip to the store, but it also guarantees you’ll always have some buttermilk ready whenever you need it.

pouring homemade buttermilk into a glass jar.

Homemade Buttermilk Substitute for Baking

Traditional buttermilk is actually the liquid left behind after churning butter, and it has a distinctly creamy and acidic flavor. The natural acid in buttermilk not only reacts with baking soda for lift, but also slightly breaks down gluten, giving baked goods a tender, soft crumb. If you’re like me and don’t always keep some on hand (those bottles are so big, and I never seem to finish them before they go bad), this substitute for buttermilk will come in handy. It delivers the same chemical reaction that many recipes depend on without the extra waste.

Regular milk doesn’t react with baking soda the way buttermilk does, so skipping the acid can lead to flatter, denser baked goods. Plus, it’s so easy to make this DIY buttermilk with just one extra ingredient. Both vinegar and lemon juice work the same way, so it’s a matter of flavor preference or what you have on hand. Let it sit for 5 minutes, and you’ll see it thicken and curdle slightly–that’s when you know it’s ready to use.

overhead view of a spoon resting in a bowl of DIY buttermilk.

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Only Make What You Need

Even as someone who bakes daily, I always recommend making only the amount of homemade buttermilk you need for your recipe. Since it takes just 5 minutes, there’s no need to store large amounts that might spoil.

featured homemade buttermilk
4.80 from 5 votes

How to Make Buttermilk

Give your baked goods the perfect touch by using this Homemade Buttermilk. You’ll learn how to prepare buttermilk from scratch so you can add it to some of your favorite recipes.
Prep Time: 5 minutes mins
Total Time: 5 minutes mins
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Equipment

  • Kitchen Scale (optional)
Serves 16 tablespoons

Ingredients

  • 1 cup milk *
  • 1 tablespoon white wine vinegar or lemon juice **
US Customary | Metric

Instructions

  • Place the milk in a cup or bowl. Remove 1 tablespoon of milk, then stir in the acid (either vinegar or lemon juice). Set it aside for 5 minutes before using.
    1 cup milk, 1 tablespoon white wine vinegar or lemon juice
    overhead view of freshly made buttermilk substitute in a glass bowl.

Notes

Yield: This recipe makes 1 cup of buttermilk.
*I recommend whole milk for the richest results, but any kind will work. 
**You can use bottled lemon juice in the milk if you don’t have fresh juice from a lemon to use.
Tips:
  • Dairy-Free Option: You can also use almond, oat, or soy milk as a dairy-free buttermilk alternative; just add the acid the same way.
  • Don’t let your buttermilk sit out for too long. It only needs about 5 minutes before you should use it or put it in a storage container and place it in the refrigerator.
  • If you need more than a cup of buttermilk for a recipe, you’ll need to double this recipe.
  • This homemade version isn’t meant for sipping (store-bought cultured buttermilk is drinkable, while this one is purely a baking ingredient). 
Storage: Store homemade buttermilk in an airtight container in the refrigerator for up to 3 days, and stir well before using. If it smells sour beyond its tangy norm, looks separated, or develops mold, it’s time to toss it.
Nutrition Facts
How to Make Buttermilk
Amount Per Serving (1 tablespoon)
Calories 9 Calories from Fat 5
% Daily Value*
Fat 0.5g1%
Saturated Fat 0.3g2%
Polyunsaturated Fat 0.01g
Monounsaturated Fat 0.1g
Cholesterol 2mg1%
Sodium 5mg0%
Potassium 21mg1%
Carbohydrates 1g0%
Sugar 1g1%
Protein 0.5g1%
Vitamin A 23IU0%
Vitamin C 0.01mg0%
Calcium 17mg2%
Iron 0.004mg0%
* Percent Daily Values are based on a 2000 calorie diet.
Author: Becky Hardin
Course: Sauce
Cuisine: American
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How to Make Buttermilk Step-by-Step

Stir and Wait: Place 1 cup of milk in a cup or bowl. Remove 1 tablespoon of milk, then stir in 1 tablespoon of acid (either vinegar or lemon juice). Set it aside for 5 minutes before using.

overhead view of freshly made buttermilk substitute in a glass bowl.

How to Store and Freeze

Homemade buttermilk will keep in the refrigerator for up to 3 days. However, it’s so simple to make that I usually just make as much as I need in the moment. If you do have leftovers, make sure to keep them in an airtight container and stir well before using. You can also freeze them in an ice cube tray, then transfer to an airtight container to store for up to 3 months. Just make sure to thaw before using.

Recipe Inspiration

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Becky Hardin Avatar

Meet

Becky Hardin
Becky Hardin has been blogging since 2012 and founded Easy Dessert Recipes in 2020. We love creating recipes that make putting desserts and sweets on the table faster and tastier for busy families. Becky has been featured in The Kitchn, People Magazine, Home and Family, The Today Show, and more.
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4.80 from 5 votes (4 ratings without comment)

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2 responses

  1. Jinx Hartigan
    November 13, 2022

    Becky, FYI your text says add a teaspoon of vinegar or lemon juice but the recipe says add a tablespoon. Many thanks for providing this so we don’t rush out to buy an unneeded carton of buttermilk!5 stars

    Reply
    1. Becky Hardin
      November 14, 2022

      Good catch! The post should be fixed now!

      Reply
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